Electroformation of Uniformly Sized Giant Liposomes with Functional Membrane Proteins
نویسندگان
چکیده
منابع مشابه
Electroformation of Giant Liposomes from Densely Micro-patterned Lipid Films
We describe a method for controllably generating giant balloon-like liposomes through electroformation of densely micro-patterned lipid films. These balloon-like liposomes are still tethered to the substrate through a thin lipid tube. We found that the patterns that were more densely spaced generated the balloon-like liposomes, whereas patterns that were sparsely spaced generated hemi-spherical...
متن کاملCa-Mediated Electroformation of Cell-Sized Lipid Vesicles
Cell-sized lipid giant unilamellar vesicles (GUVs) are formed when lipid molecules self-assemble to construct a single bilayer compartment with similar morphology to living cells. The physics of self-assembly process is only generally understood and the size distribution of GUVs tends to be very polydisperse. Herein we report a strategy for the production of controlled size distributions of GUV...
متن کاملElectroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes
Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively monodisperse, defect-free vesicles. Currently, however, it is expensive and time-consuming comp...
متن کاملWorking with liposomes and membrane proteins in Biacore systems
Solubilized proteins Detergent-solubilization of membrane proteins has proved to be a successful approach in many cases. Both ionic and nonionic detergents have been used: the choice of detergent may be crucial for retaining protein activity. Generally, detergents should be used at concentrations above their critical micelle concentration (CMC). Micelles formed above the CMC mimic a membrane en...
متن کاملElectroformation of Giant Vesicles on a Polymer Mesh
Electroformation of cell-sized lipid membrane vesicles (giant vesicles, GVs) from egg yolk phosphatidylcholine under applied electric voltage was examined on a substrate of a polymer mesh placed between two planar indium tin oxide coated glass electrodes. Under appropriate conditions, GVs were formed in good yield on meshes of various polymer materials, namely, hydrophobic poly(propylene), poly...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2014
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2013.11.3497